It is known that attitudes of physicians and motivation by community nurses influence uptake of cervical cancer screening methods by women. A cross-sectional study was conducted using a self-administered questionnaire to assess the knowledge of cervical cancer, its risk factors, beliefs about treatment and practice of screening methods among 100 primary health care workers in all primary health care centers in Ikenne LGA, Ogun State. A purposive sampling method was employed for the sampling technique. Data were analyzed using frequency tables and Pearson’s correlation. The result, showed a significant relationship between the level of education awareness cervical cancer, while there is no significant relationship between the knowledge of cervical cancer screening and practice of cervical cancer screening (Correlation is significant at the 0.01 level). Continuing medical education and programs aimed at improving knowledge of cervical cancer and its screening practice, among primary health care providers is necessary.