Prevalence and antibiotic susceptibilty pattern of Panto-valentine Leucocidin(PVL) positive Staphiloc

Prevalence and antibiotic susceptibilty pattern of Panto-valentine Leucocidin(PVL) positive Staphiloc

Author by Okon K O

Journal/Publisher: Sierra Leone Journal Of Biomedical Research

Volume/Edition: 4

Language: English

Pages: 19 - 23

Abstract

 Panton-Valentine leucocidin (PVL), a synergohymentropic  toxins encoded on S.aureus genes  are associated with soft tissue infection and community-acquired  staphylococcal infection. The purpose of the study  was to determine the prevalence and antibiotic susceptibility  of PVL-postive S.aureus isolates from clinical specimens.A total of 96 consecutive S.aureus isolates were examined. 12(12.5%) methicillin-resistant S.aureus strains(MRSA) and 84(87.5%)methicillin-sentive S.aureus(MSSA) identified by disc-diffusion and PCR assay methods. Screening of S.aureus isolates for PVL locus by PCR assay, 50(52.1%) amplified the PVL genes,  35(70.0%) were recovered  from outpatient, 15(30.0) from inpatient. PVL positive S.aureus were isolated from wound specimens, 20 (40.0%); 9(18.0%) urine, 6(12.0%) and least 1(2.0%) each from blood culture and endocervical swab. Staphylococcal cassette chromosome mec typing by two standard multiplex PCR assay, revealed an uncharacterized resistance element.  Overall antibiotic susceptibility pattern showed relatively high degree of susceptibility,however 1 isolate demostrated multidrug resistant pattern, 37(74.0%)resistant to only penicillin, 5 to one additional drug with penicillin, and 3 to two-additional drugs. The  high prevalence of S.aureus PVL-positive  strains posed dire clinical conquences, because co-existence of MRSA strains with MSSA PVL –positive strains could result in the emergence of MRSA PVL-positive


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