The effect of extracting solvents (absolute methanol, ethanol, acetone and ethyl acetate) on the phenolic,
flavonoid contents and antioxidant activities of the bark of Azadirachta indica, leaves of Acalypha wilkesiana, and
Solanum scabrum were studied. The total phenolic content (TPC) was determined using folin-ciocalteu method
while total flavonoid content (TFC) was determined using aluminum chloride method. Antioxidant activity was
determined using 2, 2-diphenyl-1-picryl hydrazine (DPPH) free radical scavenging and inhibition of lipid
peroxidation. Acetone extract of S. scabrum recorded the highest phenolic content (34.2g GAE/100g) while
methanol extract of A. indica recorded the lowest (3.77g GAE/100g). Ethanol extract of A. indica recorded the
highest flavonoid content (8.7 g QE/100g) while acetone extract of A. wilkesiana recorded the lowest (3.41 g
QE/100g). Methanol extract of A. wilkesiana recorded the highest DPPH free radical scavenging activity (85.65%)
while etanhyl acetate extract of A. indica recorded the highest inhibition of lipid peroxidation (41.57%). The result
of this study showed that the activity of antioxidant of different plants is dependent on extracting solvents.
Nature and Science 2011;9(7):53-61]. (ISSN: 1545-0740). http://www.sciencepub.net.