Aim: This study was designed to evaluate the in vivo antioxidant and hepatoprotective potentials of Costus afer stem extracts against
paracetamol induced acute liver injury.
Methods: Fifty male albino rats were randomly distributed into ten groups of five rats each and orally co-administered with varying doses
(20 - 60 mg/kg b.w.) of ethanol stem extract (ETSE), chloroform stem extract (CFSE) and paracetamol (PARA) (1 g/kg b.w.) for 7 days. In
vivo liver and plasma antioxidants and hepatotoxic biomarkers were examined by using spectrophotometric methods.
Results: Antioxidant study indicated no significant difference (p>0.05) in liver and plasma superoxide dismutase (SOD) activities in all
treated groups compared with untreated control group. Catalase (CAT) activity in co-administered “PARA + ETSE” treated groups were
significantly reduced (p<0> ETSE” treated groups were elevated compared with “PARA + CFSE” and PARA treated groups while the liver and plasma glutathione Stransferase
(GST) activities in “PARA + ETSE” treated groups were reduced compared with “PARA + CFSE” and PARA treated groups.
Liver and plasma malondialdehyde (MDA) concentrations were significantly reduced in “PARA + ETSE” treated group compared with
“PARA + CFSE” and PARA treated groups. Furthermore, “PARA + CFSE” treated groups have reduced plasma aspartate amino transferase
(AST) and alanine amino transferase (ALT) activities compared with “PARA + ETSE” treated groups and PARA treated groups.
Conclusion: This investigation indicates that C. afer ETSE possess a higher antioxidant activity than CFSE while CFSE had a higher
hepatoprotective property against paracetamol-induced tissue damage. Hence, C. afer stem could be exploited in drug discovery process to
identify potential biopharmaceutical agents against oxidative stress related diseases and hepatic disorders.
Key words: Antioxidant; Costus afer; Stem; Hepatotoxicity; Paracetamol.