In vitro antioxidant activities of ethanolic extract and triterpenoid fraction of Garcinia kola stem bark and inhibition of arsenic-induced rat liver and kidney mitochondrial membrane permeability transition.

In vitro antioxidant activities of ethanolic extract and triterpenoid fraction of Garcinia kola stem bark and inhibition of arsenic-induced rat liver and kidney mitochondrial membrane permeability transition.

Author by Dr. Godswill Anyasor

Journal/Publisher: International Journal Of Advanced Research

Volume/Edition: 5

Language: English

Pages: 724 - 733

Abstract

This study was designed to investigate the in vitro antioxidant activities and inhibitory effects of G. kola stem bark ethanolic extract (EEGK) and triterpenoid fraction (TFGK) on arsenic-induced liver and kidney mitochondrial membrane permeability transitions (MMPTs). DPPH (1-1-diphenyl 2-picryl hydrazyl), hydrogen peroxide scavenging (HPSA), hydroxyl radical scavenging (HRSA) and total antioxidant capacity (TAC) assays were performed to determine antioxidant activity in vitro. Rat liver and kidney mitochondrial fractions were isolated using differential centrifugation technique while MMPT assay was performed using spectrophotometric method. Results showed that EEGK had a significantly (P<0>


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