Anti-inflammatory and antioxidant activity of Costus afer Ker Gawl. hexane leaf fraction in arthritic rat models.

Anti-inflammatory and antioxidant activity of Costus afer Ker Gawl. hexane leaf fraction in arthritic rat models.

Author by Dr. Godswill Anyasor

Journal/Publisher: Journal Of Ethnopharmacology

Volume/Edition: 155

Language: English

Pages: 543 - 551


Ethnopharmacological relevance: Costus  afer  Ker Gawl is an indigenous tropical African medicinal
used as  therapy in the treatment of in?ammatory ailments such as  rheumatoid arthritis. This  
study was designed to evaluate the anti-in?ammatory and antioxidant activities of  the hexane
fraction of  C. afer leaves (CAHLF).
Materials  and  methods: The   anti-in?ammatory   effect of   varying  doses  of   CAHLF  on   
carrageenan, arachidonic acid, and formaldehyde induced arthritis in  male albino rats' models were
investigated in order to study the acute in?ammatory phase. Complete Freund's Adjuvant
(CFA)-induced arthritis model was used to study the chronic in?ammatory phase. Two  known
anti-in?ammatory  drugs, Diclofenac sodium (non-steroidal anti-in?ammatory  drug [NSAID])  and  
prednisolone  (glucocorticoid [steroidal drug]) were used as  standards for  comparison. Various
biochemical indices viz.  superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase
(GST), reduced glutathione (GSH)  and malondialdehyde (MDA),  aspartate amino transferase (AST),
alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB),  total protein
(TP),  globulin and albumin levels were assayed using spectrophoto- metric methods.
Results: Control  animals  in  which arthritis  have been  induced using carrageenan, arachidonic
acid, formaldehyde or CFA showed signi?cant increases (P o 0.05) in paw edema when compared with
normal animals. Treatment  of  the arthritis induced rats with CAHLF signi?cantly (P o 0.05)
suppressed the edema. in  vivo  antioxidant study showed that CAHLF treated  animals had a  
signi?cantly (P o 0.05) elevated GSH level, SOD, CAT and GST activities while MDA levels were
signi?cantly (P o 0.05) reduced in the plasma, liver, kidney and brain. CAHLF treated rats had a
signi?cantly (P o 0.05) reduced plasma AST, ALT and ALP. Plasma TP, globulin, TB levels were
reduced while albumin levels were elevated in  CAHLF treated animals.
Conclusions: CAHLF possesses substantial anti-in?ammatory  and antioxidant activities against
in?am- matory diseases especially arthritis. It could be  considered as a choice candidate in
pharmaceutical anti- in?ammatory drug development.

@ 2014 Elsevier Ireland Ltd. All rights reserved.


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