Comparative Studies of the Proteolytic and the Milk Clotting Activities in the Latex of Three Selected Plants

Comparative Studies of the Proteolytic and the Milk Clotting Activities in the Latex of Three Selected Plants

Author by Dr. Olutayo Shokunbi

Journal/Publisher: Ife Journal Of Science

Volume/Edition: 7

Language: English

Pages: 31 - 35

Abstract

The use of enzymes as catalysts for industrial processing is becoming increasingly widespread, particularly in the food industry. The proteolytic and milk clotting activities of the crude latex of Calotropis procera, Carica papaya and Musa paradisiaca were examined. The proteolytic activities of the crude latex were determined using modified casein digestion method. All the crude latex (16.7% concentration) yielded significant proteolytic activities of (5.63 ± 0.031) x 10-2 min-1, (5.29 ± 0.015) x 10-2 min-1 and (4.97 ± 0.014) x 10-2 min-1 for C. procera, C. papaya and M. paradisiaca respectively. At 16.7% concentration of the crude latex, C. procera and C. papaya had the milk clotting activities of 14.21 ± 0.72 min-1 and 6.33 ± 0.20 min-1 respectively; while M. paradisiaca yielded no milk clotting activity. However, preparation of 100% concentrated crude latex of M. paradisiaca yielded 4.81 ± 0.11 min-1 milk clotting activity. The t-tests (p<0>C. procera, C. papaya and M. paradisiaca crude latex show that their activities are significantly different from one another. Nevertheless, the respective relative activities of these three plants are significantly high enough for them to be used for either proteolytic assay and or milk clotting processes.


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