Hematological and lipid profile evaluation of a hexane fraction of Costus afer leaves in arthritic rats

Hematological and lipid profile evaluation of a hexane fraction of Costus afer leaves in arthritic rats

Author by Dr. Godswill Anyasor

Journal/Publisher: Pharmaceutical Biology

Volume/Edition: 53

Language: English

Pages: 1671 - 1676

Abstract

Context: Costus afer Ker Gawl. (Costaceae) is an ethnomedical plant used as therapy against
inflammatory disorders.
Objective: The objective of this study was to evaluate the hematological and lipid profile
analysis of hexane fraction of C. afer leaves (CAHLF) in arthritic rats.
Materials and methods: Male albino rats were randomly distributed into seven groups of six rats
each. Rats were induced with arthritis using formaldehyde and Complete Freund’s Adjuvant
(CFA) for 7 and 21 d, respectively. The animals were administered orally with 50, 100, and
250 mg/kg CAHLF, 10 mg/kg diclofenac and prednisolone, 0.9% NaCl (control), and 0.9% NaCl
(normal). At the end of treatment periods, blood samples were withdrawn and subjected to
hematological and biochemical analysis using auto-analyzer and spectrophotometric methods.
Results: Hematological analysis revealed that in formaldehyde- and CFA-induced arthritic
rat models, 250 mg/kg CAHLF-treated groups had significantly reduced (p50.05) hematocrit
counts (HC) (30.98 ± 1.59% and 33.55 ± 1.10%), white blood cell counts (WBC) (5.50 ± 0.35
and 4.15 ± 0.82109/L), and platelet counts (PC) (401.50 ± 48.94 and 246.33 ± 5.54109/L)
compared with control HC (46.90 ± 1.92 and 41.88 ± 2.19%), WBC (11.09 ± 0.26 and
7.37 ± 0.34109/L), and PC (783.67 ± 59.51 and 593.83 ± 36.3109/L). Furthermore, blood
analysis showed that CAHLF-treated groups had reduced total cholesterol, low-density
lipoprotein cholesterol, and triglycerides while they had an elevated high-density lipoprotein
compared with the control group.
Discussion and conclusion: Findings from this study indicated that CAHLF could possess
immunomodulatory and hypolipidemic properties in arthritic rats. CAHLF could be considered
as a source of biopharmaceutical agents in anti-arthritis drug discovery process.


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