A 3-week feeding trial was conducted for the biochemical assessment on the effect of Aspergillus niger (A. niger) fermented chrysophyllum albidum seed meal-based diet on growth and hematological blood parameters in rattus norvigicus weaned male albino rats. chrysophyllum albidum seeds were prepared by dehulling and subjecting to fermentation for 3 weeks. After which 18 weaned male albino rats, were randomly distributed into 3 groups, with each treatment consisting of 6 rats. The 1st group was placed on soybean meal-based diet, this served as the control, the 2nd placed on raw chrysophyllum albidum seed meal-based diet, the 3rd group on fermented chrysophyllum albidum seed meal-based diet for 3 weeks. Hemoglobin concentration was determined using the method described by Sahl (1978). Findings showed the average final live weight of rats placed on soybean meal-based diet to be 135.50±14.43, 106.00±0.07, for those fed on the raw chrysophyllum albidum seed meal-based diet and 122.33±0.22 for rats fed on fermented chrysophyllum albidum seed meal-based diet. Furthermore, hematological parameters of blood samples of rats fed on fermented chrysophyllum albidum seed meal based diet, was significantly higher (p<0>A. niger fermentation of chrysophyllum albidum seed improved the nutritional quality of the chrysophyllum albidum seed meal as evidenced in the growth and hematological parameters of experimental animals.