Revenue generation through taxation is one of the major sources of revenue open to many advanced states of the world. In the same vein, most of the second and third world nation-states have also taken bull by the horns; they have directed their energy and pherapenalia of their states toward generating revenue through efficient tax regime. However, this loft aim can only be achieved by the tax administration anchored by the tax stakeholders at all levels of governments. That is, stakeholders in the three tiers federation like Nigeria and other climes; it is all depends on the nature of the form of government adopted. Hence, the main focus of this article is to analyze the nature, responsibilities and roles of major tax stakeholders in revenue generation in Nigeria. The stakeholders can also be referred to as anchors of the revenue generation. For these roles to be effectively performed there is need for regular training and retraining of the tax officers, the expertise in the field of taxation and tax laws should be recruited to fill the emerging vacancies. The way and manner these responsibilities are performed to enhance assessment, charging, collection and remittance of same to the trios of the tiers of government are discussed in the body of this article.