Multiple shoot induction from embryo derived callus cultures of cowpea (Vigna unquiculete I.) Walp

Multiple shoot induction from embryo derived callus cultures of cowpea (Vigna unquiculete I.) Walp

Author by Dr. Foluke Odutayo

Journal/Publisher: African Journal Of Biotechnology

Volume/Edition: 4

Language: English

Pages: 1214 - 1216

Abstract

. The production of whole plant of cowpea (Vigna unguiculata) L. Walp from calli is an efficient, reliable
and rapid strategy. This provides a faster method of micro propagation. Seeds of the pea were surface
sterilized, and then the embryo axes were dissected and germinated. Callus growth was induced on the
cut surface of the embryo of V. unguiculata. Root development in the cells was promoted by the action
of the 'auxin, naphthalene acetic acid (NAA), at low cytokinin concentration. After five weeks, multiple
shoots ranging from two to four developed from the calli cultures after being subcultured on media with
high concentration of cytoklnln, benzylaminopurine (BAP). Percentages shoot production from calli
grown on media with 1 I1M concentration of BAP was 45.5% while calli subcultured on 4 !J.M BAP
produced 87.5% shoot production. Percentage rate of survival was between 21-26% in the hardened
transplanted plantlets.


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