Shool tips and meristematic tissues with latent buds were used us explunts in shoot bud culture, 111 explants were
dissected to a length of 6mm and this was stimulated to produce multiple shoots.
Basal medium was a modified Murushige and Skoog (MS) medium, which contained small amounts of auxin and
varying concentrations of cytokinin for shoot induction. The number of shoots that developed was dependent on the
amount of cytokinin-Benzyl Amino Purine (BAP). .
However it was also observed that O.OSmM concentration of BAP produced the highest rute of shoots and this was
regarded' as the optimal cytokinin concentration since' any concentration above this optimal concentration was
observed to reduce the number am! rate of shoot induction. Each multiplication stage was 8 weeks long, while
regenerntion of plnntlets ready for soil transplantation required 3·4 months,