Evaluation of the hepatoprotective effect of oral administration of aqueous fraction of methanolic extract of Costus afer leaves during induction of hepatocellular carcinoma with diethylnitrosamine in rats.

Evaluation of the hepatoprotective effect of oral administration of aqueous fraction of methanolic extract of Costus afer leaves during induction of hepatocellular carcinoma with diethylnitrosamine in rats.

Author by Dr. Godswill Anyasor

Journal/Publisher: Comparative Clinical Pathology

Volume/Edition: 29

Language: English

Pages: 733 - 744

Abstract

This study evaluated the hepatoprotective effect of oral administration of aqueous fraction of methanolic extract of Costus afer
leaves (CALAF) during induction of hepatocellular carcinoma (HCC) with diethylnitrosamine (DEN) in rats. The methanolic
leaf extract was fractionated into hexane, ethyl acetate, butanol, and aqueous fractions. The in vitro antioxidant potential of the
fractions were estimated by the assays of 2,2-diphenyl-1-picrylhydrazine and nitric oxide radical scavenging activity, ferricreducing
antioxidant potential, and total antioxidant capacity. CALAF had the most antioxidant effect. Rats were orally pretreated
daily with CALAF at 100, 200, and 400 mg/kg or silymarin (hepatoprotective drug) at 50 mg/kg from 2 weeks prior to HCC
induction and through 6 weeks of HCC induction. The HCC induction was by a single intraperitoneal injection of DEN at 200
mg/kg as an initiator, followed 2 weeks later by daily oral administration of 2-acetylaminofluorene at 30 mg/kg as promoter. At
the end of HCC induction, levels of alpha-fetoprotein (AFP), liver function and antioxidants, gamma histone 2A family member
X (?H2AX), and O6-methylguanine-DNA methyltransferase (MGMT) expressions were determined. HCC rats treated with
CALAF at all doses had significantly (p < 0> superoxide dismutase, glutathione peroxidase, reduced glutathione, and ?H2AX protein expression, whereas MGMT protein
expression was elevated when compared with untreated HCC rats. Thus, CALAF could be effective in protecting against DENinduced
HCC in rats by ameliorating hepatic injury and genotoxicity.


Other Co-Authors