Background and Objective: Cymbopogon citratus is a commonly used source of herbal medicine throughout Africa in the treatment of different kind of ailments. This study was designed to determine the anti-inflammatory activity of aqueous and hexane fractions of C. citratus leaves in vitro. Materials and Methods: The aqueous and hexane fractions were obtained from solvent partitioning of 70% methanol leaf extract and were used in standard anti-inflammatory assays, erythrocyte membrane stabilization assay, anti-denaturation assay and anti-proteinase assay using in vitro methods. Quantitative phytochemical constituents were also determined using standard quantitative methods. Results: The C. citratus leaves hexane fraction with an IC50 of 56.88, 625.00 and 480.77 ?g mL–1 showed a higher stabilization of human red blood cell (HRBC) membrane, anti-denaturation of protein and anti-proteinase activities, respectively when compared with aqueous fraction with IC50 of 89.45, 793.65 and 568.18 ?g mL–1. Phytochemical analysis of aqueous and hexane fractions of C. citratus leaves showed the presence of flavonoids, phenols, alkaloids, tannins, and saponins. Conclusion: This study therefore suggested that the hexane fraction of C. citratus leaves may be considered a conceivable basis of anti-inflammatory driver for pharmaceutical drug development.