Garcinia kola Mitigates 3, 4-methylenedioxymethamphetamine-induced Derangement in Cellular and Molecular Characterization in the Cerebellum of Wistar rat

Garcinia kola Mitigates 3, 4-methylenedioxymethamphetamine-induced Derangement in Cellular and Molecular Characterization in the Cerebellum of Wistar rat

Author by Mr. John Afees Olanrewaju

Journal/Publisher: Current Neurobiology

Volume/Edition: 112

Language: English

Pages: 37 - 47

Abstract

Background: 3, 4-methylenedioxymethamphetamine (MDMA) causes neurological disorders. Garcinia kola (GK) has neuroprotective property. We investigated the neuroprotective roles of GK on MDMA-induced cerebellar damage. Method: Sixty male Wistar rats (120 - 160g), 5 months old were grouped into six (n =10). Rats were fed with normal chow and water ad libitum. Group A was given distilled water, B and C received 100mg/kg and 200mg/kg GK respectively while D received 20mg/kg MDMA. Groups E and F received 100mg/kg and 200mg/kg GK respectively (pre-treatment), followed by intermittent 20mg/kg MDMA administration. All administrations were via oral route for 21days. Food, water intake, body temperature and weight were recorded. Rats were sacrificed and the brains were excised and used for assays. One-way ANOVA was used to analyze data followed by Student Neuman-Keauls test. Graph pad Prism 5 was used for analysis, p-values ? 0.05 were considered statistically significant. Results: Purkinje cells reduced in number and glial GFAP expression was increased. Serotonin, dopamine and glutamate levels showed no differences. MDMA treatment increased cytochrome c oxidase and glucose-6-phosphate dehydrogenase levels. Distance travelled was reduced while number of turns and foot slips were increased (p < 0>

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