Nine components (C1–C9) were isolated from chloroform fraction
of fractionated methanol extracts of Vernonia amygdalina leaves
(FMEVA) by column chromatography. All the components C1 to
C9 were purified and screened for hypoglycaemic activities in
type-2 diabetic rats. The most potent hypoglycaemic component
was elucidated on the basis of extensive spectroscopic (1D-, 2DNMR,
GC-MS, FTIR) data analysis. The Component C5 was found
to be the most potent hypoglycaemic in reducing blood glucose
by 12.55 ± 3.55% at 4 h post-oral administration, when compared
to the positive (18.07 ± 1.20%) and negative (1.99 ± 0.43%) controls.
The spectroscopic data analysis reveals that the isolated
compound has a structure consistent with 11b,13-dihydrovernolide.
The isolated compound is part of the hypoglycaemic components
present in V. amygdalina leaves that is responsible for the
anti-diabetic activities. Further research is needed in the development
of this compound or its derivatives for pharmaceutical use.