Optimization of Xylanase Production from Fermentation of Water Hyacinth (Eichhornia crassipes) using Trichoderma species.

Optimization of Xylanase Production from Fermentation of Water Hyacinth (Eichhornia crassipes) using Trichoderma species.

Author by Dr. Stanley I.r. Okoduwa

Journal/Publisher: Journal Of Biotechnology Research

Volume/Edition: 2017

Language: English

Pages: 15 - 24

Abstract

Background: In the present study, optimized cultural conditions for enhanced production of xylanase from local soil isolate of Trichoderma species, using water hyacinth as a substrate in submerged culture fermentation is presented. Method: The Megazyme assay method was used for endo 1, 4-?-xylanase using Azo-xylan (Birchwood). Results: A continuous increase in xylanase production was observed with increasing level of substrate concentration in the medium and highest production was obtained with water hyacinth at 6% w/v level. Maximum xylanase production was achieved with a pH 5.0, incubation temperature of 30ºC and agitation rate of 150 rpm. The highest production was achieved on day five of fermentation at optimum parameters under study. Result: The study showed that production of xylanase can be cost effective using water hyacinth and can be implored on large scale for industrial applications.


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