Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echis ocellatus) Venom: An in Vitro Experiment

Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echis ocellatus) Venom: An in Vitro Experiment

Author by Dr. Stanley I.r. Okoduwa

Journal/Publisher: Iranian Journal Of Toxicology

Volume/Edition: 11

Language: English

Pages: 23 - 30

Abstract

Background:The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seedkernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom.Methods:Mango seed kernel methanolic extract was evaluated in vitrofor its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus) venom. Metalloprotease portion was partially purified from the venom of E. ocellatus with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 ?mol/min/mg protein. Results:The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a Kmof 0.31 mg mL-1 and a Vmaxof 9.09 ?mol min-1. When the enzyme was incubatedwith the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with Kmvalues of 0.56 and 1.11 mg mL-1 and Vmaxvaluesof 6.67 and 4.17-?mol min-1 for 5% and 20% inhibitor concentrations, respectively. An estimated Kivalue of 0.168 mg mL-1 was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor. Conclusion:Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety


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