This study aimed at demonstrating the activities of extra virgin olive oil (EVOO) against the effect of alcohol and benzene on the liver of wistar rats. Forty-eight (48) female Wistar rats were used for this study. They were divided into eight (1-8) groups (n = 6). Group 1-control, Group 2-25% Ethanol, Group 3-200 mg/kg/b.w Benzene, Group 4-25% Ethanol + 200mg/kg/b.w Benzene, Group 5-25% Ethanol + 2ml EVOO, Group 6-200mg/kg/b.w Benzene + 2ml EVOO, Group 7-25% Ethanol + 200mg/kg/b.w Benzene + 2ml EVOO, Group 8-2ml EVOO. Animals were euthanized through cervical dislocation after the last day of administration and the liver were excised and part was fixed in formalin solution of 10 % for histological processing and the other part were homogenized for biochemical assay in phosphate buffer before centrifugation. Histological evidence led us to understand the cellular defect (hepatic degeneration) in which benzene and ethanol manifests likewise the mitigated ability of extra virgin olive oil. The relative organ weight provides information on the extent of the hepatic degeneration. Liver function test level is significantly expressed. Conclusion: Ethanol and benzene in combination cause several damage; also, they separately induce hepatic dysfunction. Extra virgin olive oil was shown to mitigate these hepatic damages.