The seed extract of Spondia mombins was used in the biofabrication of silver nanoparticles (AgNPs) in this study. Biosynthesized AgNPs was characterised using UV-Vis Spectroscopy, FTIR, FESEM, TEM, XRD and EDX. The antimicrobial efficacy of the synthesized AgNPs was evaluated against certain pathogens. The absorption spectrum peaked at 425 nm when the UV analysis was carried out, with a large peak of 3000 to 3800 cm?1 indicating different functional groups on the AgNPs surface when the FTIR analysis was conducted. An examination of FESEM and TEM showed a number of spherical particle structures varying from 10 to 50 nm. The XRD analysis also confirmed this size range. The synthesized AgNPs inhibited growth of the microorganisms used in this study. This study demonstrates that Spondia mombins’s seed extract has biomolecules that helped to bio reduce and stabilize the synthesized AgNPs, hence confirming the possibility of using Spondia mombins seeds in AgNPs synthesis.