Cancer patients experiences various unpleasant symptoms including fatigue, nausea and vomiting which could be induced by the disease or its treatment which lead to poor adaptation as a result of inadequate knowledge of self-management. This prompted the researcher to investigate the influence of gender and education on knowledge of cancer patients on self-management of selected post chemotherapy symptoms in two tertiary institutions in Lagos State, Nigeria. A descriptive survey research design was used and a sample size of 218 respondents (LASUTH-121 and LUTH-97) that have commenced chemotherapy course at the oncology clinics was recruited using total enumeration method and purposive sampling technique. A self-designed questionnaire was used for data collection, which was pilot tested through split half approach and yielded a reliability coefficient index of 0.75.Three research questions and three hypotheses were formulated. Analysis of data was done using t-test and Analysis of Variance (ANOVA) at 0.05 level of significance. Findings of the study revealed that there were significant differences between male and female level of knowledge of self-management of chemotherapy symptoms (t = 2.251, df = 216, p = .027), also there is significant difference among different educational level (f = 3.309, p = .012) with respondent that attained university/polytechnic education exhibiting higher knowledge. Significant difference were also observed between respondent selfmanagement knowledge of fatigue (p = 0.00), nausea (p = 0.01), and vomiting (p = 0.00) in the two hospital. In conclusion, gender and educational level had significant influence on the respondent’s self-management ability, and there were differences in the knowledge of selfmanagement among the respondents in the two hospitals under study. It was therefore, recommended that patients should be educated on strategies to manage post chemotherapy symptoms such as fatigue, nausea and vomiting.