This study was conducted to estimate the effect of maize endosperm type, e.g., waxy, conventional, fodder, sweet, and high oil, and maturity stage on chemical composition and in vitro fermentation characteristics of morphological fractions. Whole maize stover was separated into four fractions, including leaf blade, leaf sheath, stem and husk. The proportion of leaf blade, leaf sheath and husk, and crude protein (CP) and phosphorus (P) contents decreased, whereas the proportion of stem and fibre content increased with the increasing of maturity stage. High oil, sweet, fodder and conventional had the highest proportion in leaf blade, leaf sheath, stem and husk, respectively. Leaf blade had the highest CP and P contents and husk had the highest neutral detergent fibre content. Conventional had the highest (P<0> stem > leaf blade. The highest and lowest values of IVOMD were observed in husk and stem, respectively. The highest TVFA concentration, molar proportion of acetate and propionate were noted in high oil, waxy and sweet, respectively. The result indicated that the nutritive value of maize stover could be improved through the selection of endosperm type and appropriate maturity stage.