Objective: The study is focused on the effect ethanol and benzene on the acinar cells and extra- virgin olive oil (EVOO) as an ameliorative agent.Methods: Forty-eight Wistar rats were divided into eight groups of six animals per group: (1) Control group (a placebo of water), (2) EVOO, (3) 25% ethanol, (4) 200 mg/kg benzene, (5) 25% ethanol+200 mg/kg benzene (EB), (6) 25% ethanol+EVOO (EO), (7) 200 mg/kg benzene+EVOO (BO), and (8) 25% ethanol+200 mg/kg benzene+EVOO (EBO). The ethanol, benzene, and EVOO were administered orally.Results: All the treatment groups lost body weight except Groups 1 and 2. The histology of the pancreas showed significant (p?0.05) damage to the acinar cells of groups exposed to 25% ethanol, 200 mg/kg benzene, and EB when compared to the control, EVOO, EO, BO, and EBO. The enzymatic activities of amylase and lipase evaluated showed significant (p?0.05) decrease in EO and BO as compared to 25% ethanol, 200 mg/kg benzene, and EB-treated groups.Conclusion: There was an elevated glucose concentration in 25% ethanol and EBO as compared to control and EVOO has anti-inflammatory potential across the ameliorated groups.
Keywords: Ethanol, Benzene, Extra-virgin olive oil, Acinar cells.