Histological Assessment Of Moringa oleifera Ameliorative Activities On Lead Toxicity In The Spleen O
Authors:
OGUNSOLA Oluseyi
Publication Type: Journal article
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Abstract
Lead has been described as a most notorious and universal metallic poisoning that produces a wide range of deleterious effects on body tissues especially due to its ability to aggravate oxidative stress and alter or influence the normal activities of ions among others. The investigation explores the potential of moringa oleifera to reduce or cancel the effect of lead poisoning on the spleen- a vital lymphoid organ. A total of 24 adult Wistar rats, aged approximately 12 months were divided into four groups labeled A-D. The Group A animals were administered Moringa oleifera leaf extract only; the rationale being to observe the sole effect of the extract. The Group B animals were exposed to lead poisoning (50mg/kg body weight). Group C animals were administered lead and moringa oleifera leaf extract concurrently (50mg/kg body weight and 100mg/kg body weight respectively). Group D animals were first exposed to lead intoxication for half total time of treatment and treated with moringa leaf extract for the second half (50mg/kg body weight for lead and 100mg/kg body weight for moringa). All treatment lasted 30 days and the animals were sacrificed by cervical dislocation. The spleen was excised from each animal and processed using the routine Haematoxylin and Eosin staining technique. Photomicrographs were obtained and analysed. Lead exposure or intoxication produced deleterious effects that include reduced sizes of the splenic pulps and consequent increase in the size or area of the connective tissue cords. Moringa oleifera leaf extract at the dosage and duration of treatment employed produced both preserving and ameliorative or reparative effects.