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Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant

Authors: ONIGBINDE ADEBAYO , Bamidele Amos-tautua1

Publication Type: Journal article

Journal: Asian Journal Of Applied Sciences

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Abstract

This study assessed polyphenolic contents, antioxidant and antimicrobial activities of the solvent solutions obtained from Combretum racemosum leaf extract. The crude aqueous methanolic leaf extract of C. racemosum was partitioned into n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (n-BuOH) fractions. Antibacterial activities were determined using agar- well diffusion and agar dilution methods. In vitro free radical scavenging activity of the fractions was measured by 2, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picryl dihydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) and nitric oxide (NO) assays. MIC of the EtOAc fraction ranged from 0.63 mg/ml to 1.25 mg/ml while that of n-BuOH and DCM fractions ranged from 0.31mg/ml to 1.25 mg/ml and 0.31 mg/ml to 0.63 mg/ml respectively. The n-BuOH fraction contains highest phenolic compounds (phenol and flavonoids), followed by EtOAc, crude, hexane and DCM. Total antioxidant capacity of the fractions follows the same order as the antioxidant. NO radical scavenging activities of all the fractions showed stronger activity than that of the reference ascorbic acid (AA). The greatest antiradical activity was found in butanol fraction; IC50 for DPPH, NO and FRAP are 38.39±7.20?g/ml; 0.11±0.03 ?g/ml and 180.5203±0.86mg/ml respectively. Our findings revealed that the leaf extract of C. racemosum possesses bioactive agents that exhibit antioxidants and antimicrobials properties.

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ONIGBINDE,A. Bamidele,A. . (0000). Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant, 5 (), 1057-1057.

ONIGBINDE,A. Bamidele,A. . "Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant" 5, no (), (0000): 1057-1057.

ONIGBINDE,A. and Bamidele,A. and . (0000). Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant, 5 (), pp1057-1057.

ONIGBINDEA, BamideleA, . Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant. 0000, 5 ():1057-1057.

ONIGBINDE,ADEBAYO , and Bamidele,Amos-tautua1 . "Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant", 5 . (0000) : 1057-1057.

O.ADEBAYO & B.Amos-tautua1 , "Antimicrobial, Antioxidant Activities in vitro and Polyphenol Contents of the Leaf Extract of a Versatile Medicinal Plant" vol.5, no., pp. 1057-1057, 0000.

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