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In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens

Authors: OLAJUYIGBE Olufunmiso , & ADEOYE-ISIJOLA Morenike

Publication Type: Journal article

Journal: Acta Biochimica Polonica

ISSN Number:

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Abstract

ABSTRACT

The in vitro antibacterial activity of pure caffeine powder
and its interaction with first line antibiotic against bacterial
isolates were investigated with the macrobroth dilution
and the checkerboard assay methods. This study
showed that caffeine and the antibiotics exhibited various
degrees of antibacterial activities. While caffeine had
MICs ranging between 67.19 and 268.75 ?g/ml, chloramphenicol
was characterized by MICs between 0.98 and
31.25 ?g/ml, kanamycin — 15.63–62.5 ?g/ml, nalidixic
acid — 0.49–250 ?g/ml, erythromycin — 0.49–62.5 ?g/
ml, tetracycline — 1.99–62.5 ?g/ml and metronidazole
— 15.63–31.25 ?g/ml. Combining ½ MICs and MICs of
caffeine with the antibiotics as well as direct combination
of caffeine and the antibiotics resulted in significant
reduction of antibiotics’ effectiveness. The fractional inhibitory
concentration index (FICI) for the combination
of ½ MICs of caffeine with different antibiotics showed
antagonistic interactions with the antibiotics except kanamycin
which had additive and indifferent interactions
with caffeine. The FICI of the MICs of caffeine combined
with antibiotics showed a reduction in the number of
antagonistic interactions as chloramphenicol, nalidixic
acid and erythromycin showed some indifferent interactions
while kanamycin was the only antibiotic that
showed indifferent interaction against all the bacterial
isolates. The direct combination of caffeine and the antibiotics
resulted in significant antagonistic interactions
higher than in the case when caffeine, at the ½ MICs
and MICs, was combined with the antibiotics. Although
caffeine demonstrated significant antibacterial activity
against the selected bacterial isolates, its combination
with the selected antibiotics resulted in significant antagonistic
interactions. Caffeine should not be combined
with antibiotics as this could result in serious therapeutic
failure and, possibly, drug toxicity in vivo.

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OLAJUYIGBE,O. ADEOYE-ISIJOLA,M. . (0000). In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens, 64 (), 255-255.

OLAJUYIGBE,O. ADEOYE-ISIJOLA,M. . "In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens" 64, no (), (0000): 255-255.

OLAJUYIGBE,O. and ADEOYE-ISIJOLA,M. and . (0000). In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens, 64 (), pp255-255.

OLAJUYIGBEO, ADEOYE-ISIJOLAM, . In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens. 0000, 64 ():255-255.

OLAJUYIGBE,Olufunmiso , and ADEOYE-ISIJOLA,Morenike . "In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens", 64 . (0000) : 255-255.

O.Olufunmiso & A.Morenike , "In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens" vol.64, no., pp. 255-255, 0000.

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