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Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf.

Authors: Aleshinloye Abimbola Ololade

Publication Type: Journal article

Journal: European Journal Of Medicinal Plants

ISSN Number:

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Abstract

This study was aimed to determine the antioxidant capacity of various fractions of crude methanol
extract of Polyalthia longifolia leaves. The various fractions were obtained by successive liquidliquid
partitioning of the crude methanol extract into n-hexane, chloroform and ethyl acetate. The
flavonoid and phenolic contents as well as the 2, 2- diphenyl-1-picrylhydrazyl scavenging activity
and total reducing power of the crude methanol extract of Polyalthia longifolia leaves and its
fractions were analyzed using standard methods. From the results obtained, ethyl acetate fractionhad the highest flavonoid (571.30 mgQE/g) and phenolic (278.45 mgGAE/g) contents and the
lowest IC50 values for 2, 2- diphenyl-1-picrylhydrazyl scavenging activity (? 1 ?g/ml) and total
reducing power (0.24 ?g/ml). However, the water soluble fraction showed least values for flavonoid
content (7.22 mgQE/g), phenolic content (48.66 mgGAE/g) and highest IC50 values for 2, 2-
diphenyl-1-picrylhydrazyl scavenging activity (505.87 ?g/ml) and total reducing power (47.99
?g/ml). The flavonoid content, phenolic content and total reducing power were in the order; ethyl
acetate fraction > chloroform fraction > methanol extract > n-Hexane fraction > water soluble
fraction. However, the inhibition of 2, 2- diphenyl-1-picrylhydrazyl free radical followed the order;
ethyl acetate fraction > methanol extract > chloroform fraction > n-Hexane fraction > water soluble
fraction. In general, the antioxidant activity of the ethyl acetate fraction was the most outstanding
when compared with the crude methanol extract and other fractions. Having established the
presence of significant concentrations of pharmaceutically important phytochemicals in the
fractions of the methanol extract of the leaves especially the ethyl acetate, more evidence based
studies such as analysis of specific chemical composition and in-vivo pharmacological potential of
each fraction is imperative in order to expound more on the medicinal usability of the various
fractions of the leaves.

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Aleshinloye,A. . (0000). Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf., 21 (), 1-1.

Aleshinloye,A. . "Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf." 21, no (), (0000): 1-1.

Aleshinloye,A. and . (0000). Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf., 21 (), pp1-1.

AleshinloyeA, . Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf.. 0000, 21 ():1-1.

Aleshinloye,Abimbola Ololade , . "Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf.", 21 . (0000) : 1-1.

A.Abimbola Ololade , "Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf." vol.21, no., pp. 1-1, 0000.

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