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Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice.

Authors: UYAIABASI Gabriel , Olufunmilayo , Ibrahim , Oluwagbemiga , Umaru Millicent Ladi

Publication Type: Journal article

Journal: Nigerian Hospital Practice

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Abstract

Background: Rapid diagnostic tests (RDTs) are used for parasite diagnosis of malaria as alternative to microscopy. Most RDTs are HPR2 base kits, but their effectiveness is often compromised by false positives and prozone effect. pLDH RDTs is a viable alternative but has limited it use.

Objective: To assess the effectiveness of pLDH RDTs and the time taken for total antigen clearance after effective drug treatment.

Method: Swiss albino mice were inoculated with 0.1ml blood parasitized with Plasmodium berghei NK65 strains. Three days post inoculation; the mice were randomly placed into 4 groups of 5 animals each and treated with 10ml/kg po distil water, 30mg/kg chloroquine, 4/24mg/kg po artemether/lumefantrine and 2.1/16.8mg/kg po dihydroartemisinin/piperaquine respectively for 3 days. Assessment were done on the first 4 days and on Days 7, 14, 21 and 28, by collecting small amount of blood from the tail of the animals to prepare thick and thin slides for microscopy and testing with Acon® Malaria P.f/Pan RDTs. The sensitivity, specificity, positive predictive value and the negative predictive value were calculated.

Result: The Acon® Malaria P.f/Pan RDT was very effective in detecting malaria, with sensitivity and specificity of 93.3% and 97.2% respectively. The positive predictive value and negative predictive value were 97.3 and 93.4% respectively. Only 2 cases of false positives were recorded. pLDH antigens were cleared after one day.

Conclusion: The pLDH RDT demonstrated high efficacy and effectiveness for parasitological diagnosis of malaria. It is a viable alternative to HPR2 RDTs.

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UYAIABASI,G. Olufunmilayo,A. Ibrahim,O. Oluwagbemiga,A. Umaru,M.L . (2017). Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice., 18 (), 28-28.

UYAIABASI,G. Olufunmilayo,A. Ibrahim,O. Oluwagbemiga,A. Umaru,M.L . "Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice." 18, no (), (2017): 28-28.

UYAIABASI,G. and Olufunmilayo,A. and Ibrahim,O. and Oluwagbemiga,A. and Umaru,M.L and . (2017). Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice., 18 (), pp28-28.

UYAIABASIG, OlufunmilayoA, IbrahimO, OluwagbemigaA, UmaruML, . Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice.. 2017, 18 ():28-28.

UYAIABASI,Gabriel , Olufunmilayo,Adeyemi , Ibrahim,Oreagba , Oluwagbemiga,Aina , and Umaru,Millicent Ladi . "Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice.", 18 . (2017) : 28-28.

U.Gabriel O.Adeyemi I.Oreagba O.Aina & U.Millicent Ladi , "Assessing the effectiveness of Plasmodium lactose dehydrogenase (pLDH) based rapid test device versus standard microscopy for diagnosis of malaria in Plasmodium berghei in Mice." vol.18, no., pp. 28-28, 2017.

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