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In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation

Authors: Cassandra Bezi, ADETUNJI Oluwaseyi , & TALABI Olaoluwa

Publication Type: Journal article

Journal: Advancements In Life Sciences

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Abstract

Moringa oleifera is a well-known world herbal plant for its amazing medicinal and
nutritional properties. The effect of methanol extract of M. oleifera can be useful in managing
diseases associated with mitochondrial membrane permeability transition pore and lipid
peroxidation. Methods: Evaluation was done at varying concentrations of the methanol pods extract on mitochondrial
membrane permeability transition pore opening (swelling) and Fe2+- H2O2-EDTA (Fenton reaction)-
induced lipid peroxidation in vitro. Five male albino rats (weighed 120-250 g) were anaesthetized and
sacrificed; the liver was excised and homogenized to obtain mitochondria pellets. This study analyzed the
effect of varying concentrations of methanol pods extract of M. oleifera at 50, 150, and, 300 ?g/ml
respectively. The effects of M. oleifera varying concentration in vitro was determined using
malondialdehyde reaction quantified at 532 nm in a UV- spectrophotometer as index for lipid
peroxidation and spectrophotometric absorptions at 520 nm was observed as an index of mitochondrial
membrane permeability pore respectively. Results: Varying concentrations of methanol pods extract of M. oleifera at 50, 150, and 300 ?g/ml in the presence and absence of triggering agent (Ca2+) inhibited opening of mitochondria membrane
permeability transition pore while 0.25, 0.50 and 1.00 mg/ml inhibited lipid peroxidation induced
mitochondria of the rat liver respectively in a concentration dependent mode. Conclusion: The results suggest that methanol extract of M. oleifera pods at high concentrations (such as 300 ?g/ml and 1.00 mg/ml respectively) may inhibit mitochondrial membrane permeability transition pore opening and lipid peroxidation.

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Cassandra,E.B ADETUNJI,O. TALABI,O. . (2018). In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation, 5 (), 179-179.

Cassandra,E.B ADETUNJI,O. TALABI,O. . "In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation" 5, no (), (2018): 179-179.

Cassandra,E.B and ADETUNJI,O. and TALABI,O. and . (2018). In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation, 5 (), pp179-179.

CassandraEB, ADETUNJIO, TALABIO, . In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation. 2018, 5 ():179-179.

Cassandra,Elohor Bezi, ADETUNJI,Oluwaseyi , and TALABI,Olaoluwa . "In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation", 5 . (2018) : 179-179.

C.Elohor Bezi A.Oluwaseyi & T.Olaoluwa , "In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation" vol.5, no., pp. 179-179, 2018.

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