Antibiotic susceptibility pattern and analysis of plasmid profiles of Pseudomonas aeruginosa from hu
Authors:
ODUTAYO Foluke
Publication Type: Journal article
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Abstract
Abstract
Multidrug resistant organisms (MDROs) constitute a major public health threat globally. Clinical isolates of Pseudomonas
aeruginosa remains one of the most studied MDROs however there is paucity of information regarding the
susceptibility of its animal and plants isolates to antipseudomonas drug in Nigeria. From a total of 252 samples consisting
of plants, animals and clinical samples, 54, 24 and 22 P. aeruginosa were isolated from vegetables, animals and
clinical sources respectively. All the isolates were identified by standard biochemical methods. Antimicrobial susceptibility
testing (AST) of the 100 P. aeruginosa isolates against 7 antipseudomonal drugs was carried out by disk diffusion
method, the phenotypic detection of ESBL was done by double disk synergy test (DDST) while plasmid extraction
on 20 selected isolates based on their resistance to 2 or more classes of antibiotics was carried out by alkaline lysis
method and analysed with Lambda DNA/Hind lll marker respectively. The AST results revealed highest resistance of
91 and 55 % to ceftazidime and carbenicillin respectively while highest susceptibilities of 99 % for piperacillin–tazobactam
and imipenem were recorded in overall assay. Fifteen out of 100 isolates specifically (10) from vegetables,
(3) clinical and (2) poultry isolates showed synergy towards the beta-lactamase inhibitor indicating production of
ESBL by DDST method. Detection of plasmids was among vegetable (n = 4), poultry (n = 4), cow (n = 3) and clinical
isolates (n = 1). Plasmid profile for the selected isolates revealed 6 of the strains had one plasmids each while 5 strains
possessed 2–4 plasmids and 1 strain had 5 plasmids. The sizes of the plasmid range from <1 to ≥23kbp. Detection
of ESBL and Plasmids among the investigated isolates is suggestive of multiple interplay of resistance mechanism
among the isolates. Plants and animal isolates of P. aeruginosa harbouring multiple mechanisms of resistance is of
concern due to the danger it poses on the public health.