IN VITRO Proliferation of Plantain using Different Concentration of Auxin and Cytokinin
Authors:
ODUTAYO Foluke
Publication Type: Journal article
Journal:
ISSN Number:
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Abstract
Abstract
This study was carried out in the Tissue Culture Laboratory of Nigerian Agricultural Quarantine Service
(NAQS), moor plantation, Ibadan. The explants used were obtained from National Horticultural Research
Institute (NIHORT) and the growth parameter studied is the number of proliferated buds. MS (Murashige and
Skoog) media supplemented with 0.18mgl-1NAA + 2.3mgl-1BAP supported the growth and bud formation in
meristem culture of Musa paradisiaca cv. Agbagba during the initiation stage. After the first apical buds
appeared, the buds were transferred to MS media supplemented with different combinations of Naphthalene
acetic acid (NAA) and Benzylaminopurine (BAP) at different concentrations (0.0mgl-1NAA + 0.0mgl-1BAP,
0.1mgl-1 NAA + 3.5mgl-1 BAP, 0.18mgl-1 NAA + 4.5mgl-1 BAP, 0.26mgl-1 NAA + 5.5mgl-1 BAP). The results
obtained showed that proliferation media supplemented with 0.18mgl-1 NAA + 4.5mgl-1 BAP yielded the highest
number of buds. This study however revealed the effect of hormone in the initiation and proliferation of plantain
buds as essential and MS medium with NAA (0.18mgl-1) and BAP (4.5mgl-1) was the optimum concentration
required for the proliferation of plantain.