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. In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac

Authors: OLAJUYIGBE Olufunmiso

Publication Type: Journal article

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Abstract

The antibacterial activities of stem bark ethanolic extract of Erythrina caï¬€ra Thunb. against bacteria in diarrhoea was determined in vitro by the agar diï¬€usion and dilution, macrobroth dilution, and time-kill assay methods. The result showed that the extract produced inhibition zones ranging between 15 Â± 1.0 mm and 23 Â± 1.0 mm, and the bacteria were susceptible at concentrations ranging between â‰¤100 and â‰¤1000 Î¼g/mL. While the MICs of the extract ranged between 39.1 and 625 Î¼g/mL, and the MBCs ranged between 78.1 and 625 Î¼g/mL, the MICs of Micrococcus luteus, Proteus vulgaris CSIR 0030, Enterococcus faecalis KZN, and Staphylococcus aureus OK3 were less than 100 Î¼g/mL, and the mechanisms of antibiosis indicated that the crude ethanolic extract was highly bactericidal against the entire test bacteria isolates. In the time-kill assay, the average log reduction of the viable cell count ranged between 0.916log10 and 1.851log10 cfu/mL on incubating the bacteria for 4 h at the MICs, while the reduction ranged between 0.183log10 and 1.105log10 cfu/mL after 8 h of incubation. Incubating the bacteria for 4 h at 2 Ã— MICs resulted in the reduction of the viable cell count to between âˆ’0.264log10 and 0.961log10 cfu/mL, while the average log reduction ranged between âˆ’3.968log10 and âˆ’0.425log10 cfu/mL after 8 h of incubation with Micrococcus luteus, Proteus vulgaris CSIR 0030, and Staphylococcus aureus OK3 being the most highly aï¬€ected bacteria. The result showed that the extract exhibited broader-spectrum antibacterial activity and justifies the use of Erythrina caï¬€ra in the folkloric medicine for treating gastrointestinal infections in South Africa.

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OLAJUYIGBE,O. . (0000). . In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac, 0 (), 0-0.

OLAJUYIGBE,O. . ". In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac" 0, no (), (0000): 0-0.

OLAJUYIGBE,O. and . (0000). . In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac, 0 (), pp0-0.

OLAJUYIGBEO, . . In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac. 0000, 0 ():0-0.

OLAJUYIGBE,Olufunmiso , . ". In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac", 0 . (0000) : 0-0.

O.Olufunmiso , ". In vitro antibacterial and time kill evaluation of the Erythrina caffra Thunb. extract against bac" vol.0, no., pp. 0-0, 0000.

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