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Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal

Authors: Okunola Alabi, EDWARD BABATUNDE ESAN , Duru , OYEDELE Oluwawapelumi , Salihu G Ramatu

Publication Type: Journal article

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Abstract

ABSTRACT Environmental management of industrial solid wastes and wastewater is an important economic and environmental health problem globally. This study evaluated the mutagenic potential of automobile workshop soilsimulated leachate and tobacco wastewater using the SOS chromotest on Escherichia coli PQ37 and the Ames Salmonella fluctuation test on Salmonella typhimurium strains TA98 and TA100 without metabolic activation. Physicochemical parameters of the samples were also analyzed. The result of the Ames test showed mutagenicity of the test samples. However, the TA100 was the more responsive strain for both the simulated leachate and tobacco wastewater in terms of mutagenic index in the absence of metabolic activation. The SOS chromotest results were in agreement with those of the Ames Salmonella fluctuation test. Nevertheless, the E. coli PQ37 system was slightly more sensitive than the Salmonella assay for detecting genotoxins in the tested samples. Iron, cadmium, manganese, copper, nickel, chromium, arsenic, zinc, and lead contents analyzed in the samples were believed to play significant role in the observed mutagenicity in the microbial assays. The results of this study showed that the simulated leachate and tobacco wastewater showed strong indication of a genotoxic risk. Further studies would be required in the analytical field in order to identify and quantify other compounds not analyzed for in this study, some of which could be responsible for the observed genotoxicity. This will be necessary in order to identify the sources of toxicants and thus to take preventive and/or curative measures to limit the toxicity of these types of wastes.

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Okunola,A.A ,E. Duru,C. OYEDELE,O. Salihu,G.R . (0000). Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal, 0 (), 0-0.

Okunola,A.A ,E. Duru,C. OYEDELE,O. Salihu,G.R . "Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal" 0, no (), (0000): 0-0.

Okunola,A.A and ,E. and Duru,C. and OYEDELE,O. and Salihu,G.R and . (0000). Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal, 0 (), pp0-0.

OkunolaAA, E, DuruC, OYEDELEO, SalihuGR, . Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal. 0000, 0 ():0-0.

Okunola,A Alabi, ,EDWARD BABATUNDE ESAN , Duru,Chinwe , OYEDELE,Oluwawapelumi , and Salihu,G Ramatu . "Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal", 0 . (0000) : 0-0.

O.A Alabi .EDWARD BABATUNDE ESAN D.Chinwe O.Oluwawapelumi & S.G Ramatu , "Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Sal" vol.0, no., pp. 0-0, 0000.

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