Preliminary Bacteriological Evaluation of Abattoir Waste water Treated With Moringa Oleifera Seed Po
Authors:
ODUTAYO Foluke
Publication Type: Journal article
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ISSN Number:
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Abstract
A preliminary bacteriological evaluation of abattoir waste
water treated with powder obtained from M. oleifera was carried out.
Isolation and biochemical techniques were employed to identify
pathogenic organisms. The M. oleifera seeds were dried in the oven
at 40â°C to constant weight. They were winnowed, shelled and
grounded using sterile mortar and pestle. A sufficient powderwas
obtained using a 400mm size sieve. Before treatment a 1ml aliquot of
the waste water sample was serially diluted, the 10ⷠand 10⸠were
cultured on Nutrient agar, MacConkey agar and Eosin Methylene
Blue agar and the plates were incubated at 37±2â°C for 24 hours.
Plates containing colonies ranging from 30 – 150 were counted and
recorded at 24 hours interval for 3 days to obtain the Total Bacterial
Count (TBC), Total Coliform Count (TCC), Escherichia coli Count
(ECC) before and after treatment.